Wuhan W&Z Biotech Co., Ltd
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Cosmetic Grade Skin Repair Powder VEGF Recombinant Human VEGF Powder

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Wuhan W&Z Biotech Co., Ltd
[China]

Address
Rm3102 C2 Buliding, Zhongnan Internaitonal City, Wuluo Road, Wuhan Wuhan Hubei
Phone
86-027-87250747
Contact name
Lina

Quick Information

  • Place of Origin: China
  • :

Description


      Cosmetic Grade Skin Repair Powder VEGF         

             Recombinant Human VEGF Powder
Product Introduction:
Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF), is an important mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis.
The level of VEGF was positively correlated with blood vessel growth in tumors and the risk of tumor metastasis. Inhibition of VEGF signal transduction can inhibit the occurrence and development of a variety of tumors. It was immobilized onto solid matrix such as agarose beads for screening antineoplastic compounds.

 

 

Specifciation:
Product Name: Recombinant Human VEGF Sepharose (rh-VEGF Sepharose)
Packing details: 25 ml, 1 ml (prepacked column), 5 ml (prepacked column)
 
Capturing Molecules in interaction with VEGF165
Binding buffer: 20 mM sodium phosphate, 150 mM NaCl, pH 7.4
Elution buffer I: 100 mM sodium citrate, pH 6.0
Elution buffer II: 100 mM sodium glycine, pH 2.7
Neutralization buffer: 1M Tris-HCl, pH 9.0
 
Buffers mentioned above are needed to be prepared by customers!
1) Wash the prepacked 5 ml column with 5~10 column volumes of distilled water to remove 20% ethanol.
2) Equilibrate the column with 5~10 column volumes of binding buffer.
3) Filtrate 3~5 ml target sample through a 0.45 m filter and load the sample.
4) Wash with 5~10 column volumes of binding buffer.
5) Wash with 10 column volumes of elution buffer I and followed by 10 column volumes of elution buffer II.
6) Discard first 1/3 column volume of elution buffer II and immediately collect 1 column volume of eluant by elution buffer II and neutralize collect fractions with neutralization buffer.
7) Analyze the elute fraction to ensure that target molecule is captured (Figure 1).


Price

  • Delivery Port : Shanghai

Packaging & Delivery

  • Packing :bottles
  • Delivery Lead Time : 3~5 days for ready goods
  • Supply Ability : 10000 bottles per month

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Wuhan W&Z Biotech Co., Ltd
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